Tournaments introduced the STI501 liquid chromatography vegetable oil in tert-butyl hydroxy anisole (BHA), 2,6 - di-tert-butyl-p-cresol (BHT) name: the liquid chromatography network launched in vegetable oils tert-butyl hydroxy anisole (BHA), 2,6 - di-tert-butyl-p-cresol (BHT), measurement method of application areas: food processing, vegetable oil processing, vegetable oil, detection of anti-oxidant in order to prevent food from being oxidized and deterioration, having a wrap role of a food additives, vegetable oil and tert-butyl hydroxy anisole (BHA) Determination method: 1. Reagents; (2) Preparation of standard solution; 3 Preparation of a sample solution; 4. chromatographic conditions; 5. gradient table; 6. instrument; spectrum; antioxidants in order to prevent food oxidation and deterioration, with a role in preservation of a food additive, edible oils and fats processing, storage, and use of the process vulnerable to light, heat, enzymes, metal ions The oxidative rancidity deterioration of more susceptible to oxidative deterioration, especially rich in unsaturated double bond vegetable oil, edible oil rancidity, produce harmful substances, commonly used lipid-soluble antioxidant tert-butyl hydroxy anisole (BHA) the liposoluble phenol, 2,6 - dibutyl-hydroxy-methylphenol (BHT), tert-butyl hydroquinone (TBHQ), these types of antioxidants can be used alone may also be used in combination, which are synthetic antioxidants, which can effectively alleviate the oxidation of fat to improve the stability of the food, but all have toxic effects on the human body, its mechanism of action is more oil in the auto-oxidation process peroxide combination of the lipid oxidation process is interrupted, so as to achieve the anti-oxidation, anti-rancidity, anti-tarnishing. Excessive food antioxidants damage to human health. States food antioxidants have strict limits. Reagent methanol: HPLC grade acetic acid: HPLC grade 2 Preparation of standard solution 1mg/ml mixed standard stock solution: take tert-butyl hydroxy anisole (BHA), 2,6 - di-tert-butyl-p-cresol (BHT ) and tert-butyl hydroquinone (TBHQ) 100mg dissolved in methanol, and quantitatively to 100 ml. Standard curve working solution: Take 1mg/ml mixed standard stock solution diluted with methanol to 10ug/ml 50ug/ml 100ug/ml, 150ug/ml 200ug/ml 250ug/ml standard solution injection, the establishment of operating curves. 3 Preparation of a sample solution Accurately weigh approximately 5 g sample is taken vegetable oil (accurate to 0.001g), placed in a 15ml stoppered centrifuge tube, add 8 ml of methanol, swirl mixed 3min, placed 2min 3000r/min centrifugation for 5 min, remove the supernatant liquid in a 25ml volumetric flask, and the residue each with 8 ml of methanol extraction, the supernatant combined in a 25 ml volumetric flask with methanol to volume, shake, the 0.45um organic phase membrane filter and set aside. 4 chromatographic conditions (1) Column: the Vertex C18 column 4.6 * 150mm * 5um (2) Mobile phase: methanol (A) and 1% acetic acid solution (B) (3) Flow rate: 0.8ml/min (4) Detection wavelength : 280 nm (5) Injection volume: 10uL (6) Temperature: room temperature for 5 Gradient table Time (min) Mobile phase A (%) mobile phase B (%) 0 40 60 7.5 100 0 11.5 100 0 13.0 40 60 15.0 40 606 instrument water bath ultrasonic, high performance liquid chromatography STI501 UV detector UV501, vibrator, vacuum pumps, centrifuges, vortex mixer, analytical balance, sand core filter. spectrum detection scheme, you can call Advisory 0571-87962118 or log in directly to the website http://www.saixikeji.com 

keywords: The tournaments Release STI501 liquid chromatography vegetable oil in tert-butyl hydroxy anisole (BHA), 2, 6 - di-tert-butyl-p-cresol (BHT) method,